The PreDictor RoboColumn MabSelect PrismA, 200 µL (17549833) is a prepacked miniaturised protein A affinity chromatography column for high-throughput process development. It is packed with MabSelect PrismA resin, combining a high-flow agarose base matrix with an alkaline-stabilised protein A-derived ligand for monoclonal and bispecific antibody purification screening.
Key Features
- Prepacked 200 µL RoboColumn format, supplied as one row of eight columns for parallel chromatography screening
- Miniaturised column design, with 10.0 mm bed height, 39 mm column height, and 5 mm column inner diameter
- MabSelect PrismA protein A resin, designed for enhanced alkaline stability and high binding capacity in antibody capture workflows
- Rigid agarose resin matrix, using highly cross-linked spherical agarose with approximately 60 µm median particle size
- High dynamic binding capacity, with approximately 80 mg human IgG/mL resin at 6 min residence time and approximately 65 mg human IgG/mL resin at 4 min
- Automation-ready format, designed for automated and parallel experiments using robotic liquid handling workflows
- Defined operating limits, with flow velocity from 16 to 1000 cm/h and maximum working pressure up to 8.0 bar
- Supplied in storage solution, delivered in 20% ethanol with recommended storage at 2°C to 8°C for affinity chromatography formats
Benefits
- Supports high-throughput screening of protein A capture conditions with reduced sample consumption.
- Helps evaluate binding, elution, wash, and dynamic binding capacity conditions in parallel.
- Provides a practical scale-down format before verification and optimisation on larger chromatography columns.
- Reduces manual handling by supporting automated liquid handling and parallel experiment design.
- Enables screening work with the same MabSelect PrismA resin chemistry used in larger process development formats.
Why Choose the PreDictor RoboColumn MabSelect PrismA, 200 µL (17549833)?
The PreDictor RoboColumn MabSelect PrismA, 200 µL (17549833) is suited to laboratories developing antibody purification workflows where sample economy, parallel testing, and scale-down relevance are important. John Morris Group supplies Cytiva prepacked chromatography formats to Australian laboratories and can assist with product selection, ordering, and availability.
